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CASE NOTES


Arthrogryposis multiplex congenita in an Angus calf

Steph Ferguson, final year student Charles Sturt University, Wagga Wagga and Bruce Watt, Central Tablelands Local Land Services, Bathurst, NSW

Posted Flock & Herd December 2021

INTRODUCTION

In 2008, a new form of arthrogryposis, characterised by a low birthweight and multiple joint involvement (and therefore referred to as arthrogryposis multiplex or AM) emerged in the Angus breed following the widespread use of the elite sire GAR Precision 1680 and his descendants, most notably CA Future Direction 5321 (Windsor et al., 2011).

A commercial Angus producer in the Bathurst region saw three small, premature but not noticeably abnormal calves during the last five years including one in 2020. The first calf born in 2021 was also small and premature but was not obviously abnormal. When a second, clearly abnormal calf was born the owner decided to investigate. The calf was small with arthrogryposis. Genetic testing was consistent with the calf having two copies of the arthrogryposis multiplex (AM) gene.

While AM has largely been eliminated in the seed stock herd though genetic testing (Denholm 2017), it is undoubtedly still present in commercial herds. Commercial breeders who use known AM-free bulls over heterozygous cows should not see affected calves but those who breed their own bulls may be at risk unless they test either the bull, breeding cows or the bull progeny.

CASE REPORT

Several small premature calves have been born on this property over the last five years. Some of these may have had AM but this hypothesis is unproven. The known affected calf (figure 1) was delivered by a two-year-old heifer on 20 August 2021. The sire of the affected calf was a homebred bull bred by AI from a known AM-free sire (Baldridge Download Z013) indicating that the dam was heterozygous for AM. Both the heifer’s sire and dam are of unknown AM status, but the homebred sire presumably also carries the gene.

The property runs 80 Angus cows and heifers, and calves from early August to mid-September. The cattle graze pastures predominately comprised of cocksfoot (Dactylis glomerata), phalaris (Phalaris aquatica), ryegrass (Lolium perenne) and subterranean clover (Trifolium subterraneum). Blue heliotrope (Heliotropium amplexicaule) is an emerging problem. When pasture production is inadequate, the cattle are supplementary fed primarily oaten hay. Calves are weaned in April and the steer portion sold, with heifers retained. The cows are vaccinated with 5-in-1 clostridial vaccine at pregnancy diagnosis in mid-April. Young stock are vaccinated against clostridial diseases (5-in-1) and infectious bovine keratoconjunctivitis (Piliguard, Coopers Animal Health) at calf marking and given a 5-in-1 booster at weaning.

NECROPSY FINDINGS

The affected calf was a 14 kg female delivered close to term with a grey hair coat and a crown rump length of 60cm. The head appeared normal with no cleft palate. There was marked scoliosis with cervical vertebrae twisting to the right and marked flexion of all joints of the left forelimb. The right forelimb had marked flexion of the shoulder and elbow, with mild flexion of the carpus and metacarpus. Additionally, there was diffuse muscular atrophy across the thorax and pelvis. The left hindlimb was markedly flexed across all joints, whilst the right hindlimb had marked flexion of the coxofemoral and stifle joint. An ear tip sample was submitted for testing for pestivirus and AM and thoracic blood was collected to test for immunoglobulins and serology if warranted.

Image of dead black calf with limb and bodily distortion
Figure 1. Affected calf with marked flexion of limbs and scoliosis

LABORATORY FINDINGS

Genetic testing of the tissue sample submitted was consistent with the calf having two copies (affected) of the mutation responsible for Arthrogryposis multiplex congenita in Angus cattle. The calf was negative for pestivirus antigen and IgG levels were not elevated.

DISCUSSION

Arthrogryposis is a congenital condition characterised by ankylosis of multiple limbs (Zachery & McGavin, 2012). The most common presentation involves marked flexion of the forelimbs, scoliosis, muscular atrophy and occasionally central nervous system signs (Windsor et al., 2011; Zachery & McGavin, 2012) The pathogenesis of the disease in Australia is complex and may result from gestational arbovirus infections or plant teratogens. A genetic form of the disease presenting as arthrogryposis of multiple joints, scoliosis, cleft palate and kyphosis has been described in Charolais and Angus cattle (Leipold et al., 1969; Romero et al., 2020). The most recent form, Arthrogryposis multiplex congenita in Angus cattle is also an autosomal recessive genetic disorder (Windsor et al., 2011).

Calves with arthrogryposis are typically stillborn or die shortly after delivery. Dystocia is common due to the musculoskeletal abnormalities associated with the condition (Zachery & McGavin, 2012).

In this case, genetic testing indicated that this calf had two copies of the autosomal recessive AM gene. Akabane infection was not tested for but was not regarded as a consideration because there were no reports of transmission during the pregnancy of the dam.

This finding indicates that there are AM-carrier cows and homebred bulls within the herd. The owner plans on testing homebred bulls prior to joining and will test potential bulls at calf marking. It is not considered necessary to test the cows to be artificially inseminated as these vary from year to year.

ACKNOWLEDGEMENTS

We would like to thank and acknowledge the owner, Ian Hendry, who provided the material and information for this case and who greatly assisted Steph Ferguson in the difficult task of completing a remote case investigation.

REFERENCES

  1. Agerholm, J. S., McEvoy, F. J., Menzi, F., Jagannathan, V., & Drögemüller, C. (2016, Jun 30). A CHRNB1 frameshift mutation is associated with familial arthrogryposis multiplex congenita in Red dairy cattle. BMC Genomics, 17, 479 doi.org
  2. Anderson, D. E., Desrochers, A., & St. Jean, G. (2008, 2008/11/01/). Management of Tendon Disorders in Cattle. Veterinary Clinics of North America: Food Animal Practice, 24(3), 551-566 doi.org
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  4. Denholm L (2017). Genotype disclosure in the genomics era: roles and responsibilities. Australian Veterinary Journal 95 (9), 308-316
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  6. Gardiner, D. J. (2009). Bovine heritable arthrogryposis multiplex congenita (BHAMC). Flock and Herd, posted 2009 www.flockandherd.net.au
  7. Kirkland, P. D. (2015, Aug). Akabane virus infection. Rev. Sci. Tech., 34(2), 403-410 doi.org
  8. Leipold, H. W., Cates, W. F., Radostits, O. M., & Howell, W. E. (1969, Oct). Spinal dysraphism, arthrogryposis and cleft palate in newborn charolais calves. Can. Vet. J., 10(10), 268-273
  9. Leipold, H. W., Huston, K., Guffy, M. M., & Noordsy, J. L. (1967). Spastic paresis in beef shorthorn cattle. Journal of the American Veterinary Medical Association, 151(5), 598-601
  10. Romero, A., Briano, C., & Quintela, F. D. (2020). Arthrogryposis multiplex congenita in Aberdeen Angus cattle in Uruguay. Pesquisa Veterinária Brasileira, 40, 426-429
  11. Shupe, J., Binns, W., James, L., & Keeler, R. (1968). A congenital deformity in calves induced by the maternal consumption of lupin. Australian Journal of Agricultural Research, 19(2), 335-340 doi.org
  12. Windsor, P., Kessell, A., & Finnie, J. (2011). Neurological diseases of ruminant livestock in Australia. V: congenital neurogenetic disorders of cattle. Australian Veterinary Journal, 89(10), 394-401 doi.org
  13. Zachery, J. F., & McGavin, M. D. (2012). Pathologic Basis of Veterinary Disease (S. Stringer, Ed. 5th ed.). Elsevier

 


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